Journal: Cancer Research

Article Title: Identification of Their Epitope Reveals the Structural Basis for the Mechanism of Action of the Immunosuppressive Antibodies Basiliximab and Daclizumab

doi: 10.1158/0008-5472.can-06-3919

Figure Lengend Snippet: Figure 4. Model of the IL-2R binding to IL-2. Magenta, IL2; yellow, contact site of IL-2Ra and IL-2; red, basiliximab epitope. E116 and V122 mark the first and the last amino acid of the epitope. Orange, overlap of IL-2 binding and basiliximab binding amino acids within the IL-2R. The model was established using the software Cn3D 4.1 based on the published crystal structure (6).

Article Snippet: After 24 h, cells were stained using basiliximab at 10 Ag/mL or a polyclonal goat anti-human IL-2Ra antibody (R&D Systems) at 7 Ag/mL for 30 min.

Techniques: Binding Assay, Software

Journal:

Article Title: A low intraepithelial effector (CD3+):regulatory (FoxP3+) T-cell ratio predicts adverse outcome of human colon carcinoma

doi: 10.1053/j.gastro.2009.06.053

Figure Lengend Snippet: T Lymphocyte Marker Localization and DNA Mismatch Repair (MMR) Status.

Article Snippet: For CD25, slides were incubated in Sniper Background Reducer (Biocare Medical) for 5 min., and then incubated with an anti-human CD25 antibody (R&D Systems) at 1:200 for 30 min. Incubation in Mach 3 Mouse-HRP polymer kit (Biocare) was performed for 15 min. Color was developed using DAB and then counterstaining with hematoxylin.

Techniques: Marker

Journal: Cancer Immunology, Immunotherapy

Article Title: Regulatory T cells promote glioma cell stemness through TGF-β–NF-κB–IL6–STAT3 signaling

doi: 10.1007/s00262-021-02872-0

Figure Lengend Snippet: Regulatory T cells (Tregs) promote glioma stem cell (GSC) capacity in glioma cells. a Distribution of CD4 + CD25 + Foxp3 + Tregs in peripheral blood and tumor samples from glioma patients ( n = 50), analyzed the percentage of total lymphocytes using flow cytometry. b CD133 , SOX2 , NESTIN , ALDH1A , and MUSASHI1 expression in the U251 cells, detected using reverse-transcription PCR, following co-culture with Tregs (1:1; Transwell) sorted from the peripheral blood of the glioma patients. c CD133 + proportions of U251 cells, assessed by flow cytometry, following co-culture with Tregs for 24 h in transwell chambers. d U251 cells and Tregs co-cultured under sphere-forming conditions. The number of spheres per field was averaged from five randomly selected fields. Results are based on three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001. NC normal control

Article Snippet: After isolation of CD4 + T cells, CD25 cells were purified using the anti-Human CD25 Biotinylated Antibody (R&D Systems).

Techniques: Flow Cytometry, Expressing, Reverse Transcription, Co-Culture Assay, Cell Culture, Control